Interesting GFP fusion protein problem with diffusion

Claire Brown Claire.Brown at curie.fr
Mon Nov 22 06:49:56 EST 1999


Sean,

I have been working with a number of GFP fusion proteins in epithelial
cells and some localize to the nucleus and some do not. We have not
investigated things any further on this aspect because we are more
interested in proteins at the brush border of these cells. However, we have
also expressed GFP alone not fused to any specific protein and we get high
levels of cytoplasmic staining. However we also see nuclear staining.

I hope this is helpful.

Claire

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Claire M. Brown                           E-mail:   Claire.Brown at curie.fr
Postdoctoral Fellow                       Phone/Fax: 33-1-42-34-67-95
Laboratoire de Physico-Chimie
Institut Curie - CNRS
11, rue Pierre et Marie Curie
75005 Paris
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