quenching fluorescence

Arjun.G. arjung at ncbs.res.in
Thu Nov 25 11:08:32 EST 1999


Hello.
Could you suggest reagents that might be used on living cells to
quench Cy3 fluorescence? I am looking at endocytosis and would like to
quantitatively address the contribution from surface bound (uninternalized)
probe. Quenchers that can cross membranes will quench all fluorescence and
will not allow me to measure the amount that remains surface bound or the
amount that is internalized.

Thanks.
Arjun.
_____________________________________________________________________________
_____________________________________________________________________________

Arjun Guha,
National Centre for Biological Sciences,
University of Agricultural Sciences, GKVK Campus,
Bangalore 560 065.

Phone #: 8561658, 8561659 extn 4260/4261
E-Mail:  arjung at ncbs.res.in
	 arjun_guha at hotmail.com
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