novice on FRET

[Oliver Schäfer]

Hello,

has someone anytime done Fluorescence Energy Transfer (FRET)? I've read that
mostly you can observe reduction in donor emission but what I need is a
strong increase in acceptor emission. It would be best if the acceptor
wouldn't emit anything without the donor. Can somoeone give me a tip for a
suitable pair of dyes?



Thanks
Oliver


Oliver Schaefer
Heinrich-Heine-Universitaet Duesseldorf
Germany