novice on FRET

Oliver Schäfer schaefer at biophys.uni-duesseldorf.de
Fri Oct 1 08:00:51 EST 1999


Hello,

has someone anytime done Fluorescence Energy Transfer (FRET)? I've read that
mostly you can observe reduction in donor emission but what I need is a
strong increase in acceptor emission. It would be best if the acceptor
wouldn't emit anything without the donor. Can somoeone give me a tip for a
suitable pair of dyes?



Thanks
Oliver


Oliver Schaefer
Heinrich-Heine-Universitaet Duesseldorf
Germany





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