Kathryn Sunn k.sunn at
Mon Oct 11 00:52:34 EST 1999

Dear All

Has anyone done a lot of colocalisation studies using GFP tagged
transfected constructs and a red-tagged antibody? If so has anyone ever had
the problem of the GFP green signal losing its fluorescence after
permeabilisation? Does anyone know any handy hints to get around this at

Thanks for your help


Kathryn Sunn  BSc (Hons)
Postgraduate Student
Bone and Mineral Research Program
Garvan Institute of Medical Research
384 Victoria St, Darlinghurst
St Vincent's Hospital                          Tel:   (02) 9295 8260
Sydney NSW 2010                                Fax:   (02) 9295 8241
Australia                          email: k.sunn at

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