eGFP quantification

Yuval Cohen yuval.cohen at curie.u-psud.fr
Tue Oct 12 20:22:15 EST 1999


I have constructed an eGFP fusion protein and transformed it into
S.cerevisiae. I now wish to use it as an alternative for lacZ for
quantifying the expression of my protein. What techniques are involved?
Shold the cells be lysed or not? What wavelength is ideal for measuring
eGFP?

Thanks in advance

Yuval





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