E.coli or enteric with a marked main chromosome

Dean Harapas Dean.Harapas at nre.vic.gov.au
Thu Oct 14 19:56:37 EST 1999



I wish to distinguish E.coli applied to vegetable plants from indigenous
and extraneous E.coli.

I have  a Clontech ampicillin and GFP plasmid, but it is lost when the
cells go into stationary phase. Do you know where I could get an E.coli (or
some enteric bacterium) with a marker engineered into the main chromosome
that isn't lost in stationary phase? The marker could even be just a
sequence, which could be detectable by hybridisation. I look forward to a
reply, even in the negative. Thanks.

Dean Harapas
Scientist
Agriculture Victoria
Institute for Horticultural Development
Private Bag 15, South Eastern Mail Centre, Victoria,  Australia 3176

Local Telephone:  (03) 9210 9222
Local Fax: (03) 9800 3521





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