Thermostable GFP for anaerobes

Paul Kitts kitts at NCBI.NLM.NIH.GOV
Fri Oct 15 10:20:24 EST 1999


Hi Sunil,

Formation of the GFP chromophore requires oxygen and so GFP will not fluoresce 
in anaerobes.

-=Paul=-

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"  Paul Kitts, Ph.D.                                           "
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"  NIH/NLM                                                     "
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To: fluorpro at net.bio.net
From: sgdksdtsd at yahoo.com (sunil desai)
Subject: Thermostable GFP for anaerobes
Date: 14 Oct 1999 09:56:40 -0700

Hi,

I am looking for a suitable reporter gene system(other
than lacZ and CAT) that can work in gram-positive,
anaerobic bacteria in the temperature range of 30 to
65 degrees celsius. I have read variant GFPs can be
used but no information is available on the exact
vector names, company that sells the product etc.
Greatly appreciate if anyone can provide more
information.

Thanks, Sunil





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