HELP:about apoaequorin detection

Paul Kitts kitts at NCBI.NLM.NIH.GOV
Thu Sep 23 16:12:21 EST 1999


Firefly luciferin will not work.
I think Molecular Probes sells coelenterate luciferin.
-=Paul=-

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"  Paul Kitts, Ph.D.                                           "
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"  NIH/NLM                                                     "
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To: fluorpro at net.bio.net
From: nsxia at jingxian.xmu.edu.cn (nsxia)
Subject: HELP:about apoaequorin detection
Date: 22 Sep 1999 20:21:38 -0700


Hi all,
We have cloned the apoaequorin gene from a kind of jellyfish and
expressed it in E.coli, now I want to detect its bioluminescent
activity, but I don't know if I can use firefly luciferin instead of
coelenterate luciferin, because I cannot find any commercial resources
for the coelenterate luciferin. Does anyone have some suggestion about
it? Any advice would be very appreciated.

Thanks in Advance

Zhang,Jun
The State Lab for Tumor Cell Engineering
Xiamen University
Xiamen,Fujian,361005
P.R.China





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