acetoxymethylester hydrolysis in yeast

Patrick Bradshaw bradshaw.23 at postbox.acs.ohio-state.edu
Tue Sep 28 00:33:17 EST 1999


  
 We are interested in loading the AM ester forms of Fura-2 or Indo-1 in the
  yeast, Saccharomyces cerevisiae.  We were not successful when we tried and 
found in the literature that others could only load yeast with the 
pentapotassium forms of Indo-1 or Fura-2 by electroporation or at very low pH. 
 Does anyone know the reason for this failure to load the AM ester forms of 
these dyes?  Do yeast lack the esterase to cleave the AM esters or are these 
dyes  impermeable to the yeast cell wall ?


                                                                                                Patrick Bradshaw
                                                                               
                 



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