FRET or BRET between DsRed and GFP or Renilla luciferase ?

Holger Fehr fehr at biozentrum.uni-wuerzburg.de
Thu Aug 3 06:08:51 EST 2000


Hi,

I wanted to measure protein-protein interactions using Clontech´s DsRed in
FRET- or BRET-assays, but havn´t see much effects so far. So my question is: 

Had anyone seen significant FRET between GFP and the Red-FP or BRET between
Renilla luciferase and RFP ? 

I didn´t see much effects even with a GFP-RFP fusion protein, but I can not
rule out that this might be a specific problem of my construct. 

So if anyone has positive or negativ proof for FRET or BRET using RFP,
please send a comment.


Sincerly,

Holger Fehr


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Dr. Holger Fehr
Inst. Physiological Chemistry II
University of Wuerzburg
Germany

fehr at biozentrum.uni-wuerzburg.de 



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