dsred vectors intensity

Frank O. Fackelmayer Frank.Fackelmayer at uni-konstanz.de
Tue Feb 8 11:24:56 EST 2000


royer wrote:

>         Has anybody an idea on intensity of dsred vectors compared to egfp
> vectors used in vivo. What conditions (filter used, ...) gain optimal
> detection of the signal? Thanks for your response. Corinne Royer
> ROYER corinne
> Unité Nationale Séricicole
> INRA
> 69350 La Mulatière
> France
>
> ---

Hi Corinne,
We´re just working with dsRed and EGFP constructs of our protein of interest,
as well as the empty vectors. We find that dsRed is **WAY** weaker that EGFP,
and it usually takes twice the time after transfection to see a good signal.
It is difficult to estimate the factor  between red fluorescent protein and
EGFP in cells, but I´d say it is at least 10. We use a standard rhodamine
filter set.

Frank





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