dsRFP detection

MILLINGTON, Michelle [JJRAU] MMILLING at MEDAU.JNJ.com
Wed Feb 16 18:46:55 EST 2000


Hi,

Just a query about Clontech's dsRFP......

We would like to use it in combination with EYFP and are wondering if anyone
has had any experience with it.  Has anyone been able to detect it by flow
cytometry using the conventional filters for PE (FL-2)???  

We have tried EBFP but are unable to detect by flow due to  low fluorescence
and photobleaching.  Is dsRFP brighter and more resistant to photobleaching
than EBFP?

Thanks for any input,

Michelle 


Michelle Millington
School of Physiology & Pharmacology
University of New South Wales
Sydney  NSW  2052
Australia


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