dsRFP detection

[Gregers]

We have tried but couldn't detect DsRed by flow cytometry. 
DsRed probably isn't excited enough at 488 nm. 

Gregers J. Gram
Laboratory for Infectious Diseases 144
H:S Hvidovre Hospital, Denmark
www.inflab.suite.dk/
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----- Oprindelig meddelelse ----- 
Fra: "MILLINGTON, Michelle [JJRAU]" <MMILLING at MEDAU.JNJ.com>
Til: <fluorpro at hgmp.mrc.ac.uk>
Sendt: 17. februar 2000 00:46
Emne: dsRFP detection


> Hi,
> 
> Just a query about Clontech's dsRFP......
> 
> We would like to use it in combination with EYFP and are wondering if anyone
> has had any experience with it.  Has anyone been able to detect it by flow
> cytometry using the conventional filters for PE (FL-2)???  
> 
> We have tried EBFP but are unable to detect by flow due to  low fluorescence
> and photobleaching.  Is dsRFP brighter and more resistant to photobleaching
> than EBFP?
> 
> Thanks for any input,
> 
> Michelle 
> 
> 
> Michelle Millington
> School of Physiology & Pharmacology
> University of New South Wales
> Sydney  NSW  2052
> Australia
> 
> 
> ---
> 

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