dsRFP detection

Beat Ludin bludin at lis.ch
Thu Feb 17 06:42:06 EST 2000


>We have tried but couldn't detect DsRed by flow cytometry.
>DsRed probably isn't excited enough at 488 nm.
>
>Gregers J. Gram
>Laboratory for Infectious Diseases 144
>H:S Hvidovre Hospital, Denmark
>www.inflab.suite.dk/
>------------------------------

Excitation efficiency of DsRed should still be 30-40% at 488nm. So if 
you can't see anything at that wavelength, it means that you would at 
best get a very bad S/N when exciting at the optimal wavelength. Is 
your emission filter (and dichroic mirror, in case there is one in 
the system) suitable for DsRed?

Beat


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