dsRFP detection

Jered Floyd jered at mit.edu
Thu Feb 17 13:47:41 EST 2000


> We´ve also used RFP and EGFP, but with reverse result: EGFP is MUCH
> brighter the RFP, at least in COS cells transfected with vectors
> dsRed-N1 and pEGFP-N1, respectively.

Of course, you are correct, and looking at the information on quantum
yield of the two proteins easily confirms this.  I just realized that
I was comparing the visible expression of DsRed vs. GFP(LVA); not a very
fair comparison at all! (and photon for photon, GFP will appear another
6 times or so as bright given the properites of the human eye.) My 
mistake.

--Jered




More information about the Fluorpro mailing list