Fluorescent imaging

[Yasuo Niwa]

Hi Jens,

I am using a FluorImager (Molecular Dynamics/Amersham Pharmacia Biotech)
to detect sGFP(S65T) expression in plants.
The sGFP(S65T) is almost the same as EGFP.
It works fine.
If you are interested in, please check my paper.

Y. Niwa et al., Non-invasive quantitative detection and applications
of non-toxic, S65T-type green fluorescent protein in living plants.
Plant J. (1999) 18, 455-463.

The FluorImager also works fine to detect EGFP expression in animal cells.

Yasuo Niwa
Univ. of Shizuoka

Jens Tornoe$B$5$s$,(J00.2.17 0:52 AM$B$K=q$-$^$7$?!'(J
>Hi all
>
>My department is about to buy a phosphorimager with fluorescent
>abilities such as the FLA-3000 from FUJI. I would like to use
>the imager to screen EGFP expressing cells, but we have not been
>able to get ant specific information about EGFP detection from
>FUJI or other fluorescent imager producers. The FLA-3000 can be
>equipped with a 473nm laser, which in my opinion should allow
>for EGFP detection.
>
>ANY comment on the matter would be highly appreciated. The sales
>representatives we have been in touch with haven't been excactly
>bursting with knowledge on the subject...
>
>Thanks
>
>Jens Torn$BF(J
>Dept. of Molecular Biology
>NsGene, Denmark
>
---