storing GFP expressing cells

Wolfgang Schechinger Wolfgang.Schechinger at med.uni-tuebingen.de
Fri Feb 18 08:38:48 EST 2000


Hi all, 

I think of storing some transfected cells (293 fibroblasts) expressin 
GFP as a reporter for some days at -20 degC.
Spontaneously, I'd resuspend them in say 20% glycerol / PBS to 
prevent the formation of ice crystals.
Later, I would dilute the solution and analyze the cells with a 
FACS.
Does this sound reasonable?
Any better suggestions are higly welcome!

Wolfgang
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Dr. Wolfgang Schechinger, Dept. of Pathobiochemistry
University of Tuebingen, Germany
email: wolfgang.schechinger at med.uni-tuebingen.de 
wwWait: http://www.medizin.uni-tuebingen.de/~wgschech/start.htm
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