DNA concentration using GFP?
clarosa at biocomp.unl.edu
Fri Jan 7 13:50:42 EST 2000
Your are confused,,,,, there is a fluorescent dye called Picogreen. you
add the sample and the dye and you need a fluorimeter. Check out
biorad, perkin elmer etc for equipment and reagents needed. GFP has
nothing to do with dna conce measurements.
Andrew Louka wrote:
> Hello everyone,
> I've heard that it's possible to use GFP and a 96-well plate
> fluorescence detector (e.g. Victor) to determine the concentration of
> purified genomic DNA. I heard that this method was used when one has
> very little sample i.e. too little to put onto a spectrophotometer.
> Can anyone tell me how this is done?
> How accurate is it compared to spec. analysis?
> Many thanks,
> Institute of Immunology
> University of Oslo, Norway
> WWW: http://www.sequenceanalysis.com/
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