clontech GFP Antibody

Cornelius Krasel krasel at wpxx02.toxi.uni-wuerzburg.de
Mon Jan 10 07:57:58 EST 2000


Kathryn Sunn <k.sunn at garvan.unsw.edu.au> wrote:
> I was wondering if anyone has used the Clontech GFP polyclonal antibody and
> achieved a clean western blot?

Yes. We used the dilutions as suggested by clontech. As secondary, we
used a peroxidase-coupled anti-rabbit from Dianova (I think they re-sell
Jackson research antibodies) in a dilution of 1:3000. Development was
with ECL.

--Cornelius.

-- 
/* Cornelius Krasel, U Wuerzburg, Dept. of Pharmacology, Versbacher Str. 9 */
/* D-97078 Wuerzburg, Germany   email: phak004 at rzbox.uni-wuerzburg.de  SP4 */
/* "Science is the game we play with God to find out what His rules are."  */




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