postdoctoral position

vonarnim at vonarnim at
Tue Jan 18 09:40:14 EST 2000

Postdoctoral Position in Protein Interactions
Vanderbilt University
Nashville, Tennessee, USA

A postdoctoral position is available April 1, 2000 to develop a new
technique for assaying protein-protein interactions in situ. This technique
assays the proximity of fusion proteins by Bioluminescence Resonance Energy
Transfer (BRET). Preliminary studies characterizing the BRET technique in
bacteria are described in:

Xu, Y., D. Piston, and C.H. Johnson. 1999. A bioluminescence resonance
energy transfer (BRET) system: Application to interacting circadian clock
proteins. Proc. Natl. Acad. Sci. USA 96: 151-156.

The candidate will focus on extending the BRET technique to mammalian cells
in culture. Because our lab primarily studies the molecular and cellular
basis of circadian (daily) rhythmicity, part of the project will also
utilize the technique to examine the interaction of circadian clock
proteins over the daily cycle in situ. Salary will be consistent with the
NIH scale. Support for this position comes from an NIH grant that begins
April 1, 2000 (pending final approval of a grant proposal that received a
high priority score) and ends March 31, 2002, but the position will be
continued if the grant is renewed and/or the candidate can apply for their
own postdoctoral fellowship after beginning the project. Required:
experience with molecular genetic techniques. Desirable: experience with
transfection of mammalian cells in culture, other protein interaction
assays, and/or microscopic techniques. Interested applicants should send a
current CV including the names of three references to: Dr. Carl Johnson,
Dept. of Biology, Box 1812-B, Vanderbilt University, Nashville, TN  37235

Please reply to the following address:

email: carl.h.johnson at


More information about the Fluorpro mailing list