Quantitating GFP expression

Cornelius Krasel krasel at wpxx02.toxi.uni-wuerzburg.de
Thu Jan 20 10:32:11 EST 2000


Jack Vanden Heuvel <jpv2 at psu.edu> wrote:
> Can GFP expression be quantitated by fluorescence spectroscopy?

I don't know but I would assume "yes".

> Do GFPs fluoresce in solution (i.e. in lysed extracts)?

It did fluoresce in our hands. We tried both GFP alone and GFP fusions
and got nice fluorescence spectra in cell lysates (it's important to
centrifuge the lysates, otherwise you will have troubles with particles
floating around).

> Any idea about the senstivity of spectroscopy versus doing western blots
> with anti-GFP antibodies?

IMO, Western blotting is much more sensitive.

--Cornelius.

-- 
/* Cornelius Krasel, U Wuerzburg, Dept. of Pharmacology, Versbacher Str. 9 */
/* D-97078 Wuerzburg, Germany   email: phak004 at rzbox.uni-wuerzburg.de  SP4 */
/* "Science is the game we play with God to find out what His rules are."  */




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