Green Fluorescent Protein Purification Short Course

gfp-1 meton at rci.rutgers.edu
Mon Jul 17 20:09:14 EST 2000


I have been asked to announce the following,

                  The State University of New Jersey
                                Rutgers
                        Campus at New Brunswick

             Center for Research and Education in Bioluminescence
                          and Biotechnology
                   Programs in Biotechnology, Presents:

Protein Purification: Isolation, Analysis, and Characterization of GFP,
A Five and One-Half Day Hands-On Laboratory Course Using the remarkable
Green-Fluorescent Protein (GFP), A Novel Marker For Gene Expression,
as the source material
                             July 23-28, 2000
                     and July 30-August 4, 2000

More than 600 scientists from around the world have strongly recommended
this
intensive course as an opportunity to develop protein research and
analytical
skills in a retreat setting.  Participants work hard, identify and solve
problems in the lab and enjoy camaraderie and good food and beer with
colleagues.

This five and one-half day laboratory course covers a wide variety of
conventional methods for protein isolation, purification, and
characterization.  The course format integrates hands-on laboratory
exercises
with classroom lectures, demonstrations, study breaks, and short
take-home
assignments.

A special feature of the course is that all laboratory work will be
performed
on the same starting sample (Aequorea GFP*), which will be purified from
an
exceedingly crude form to near homogeneity as judged by high performance
liquid chromatography (HPLC), SDS gel electrophoresis, isoelectric
focusing,
and capillary zone electrophoresis. This feature provides a continuity
of
purpose, integrating dozens of preparative and analytical protein
techniques
in a way that few competing courses can match.

A problem-solving approach will be used throughout the course.  Under
the
guidance of experienced lab instructors, participants will work in
groups
of three to plan their own protocols, analyze data, and interpret
results.
A student-teacher ratio not greater than 8:1 will be maintained and the
faculty coordinators will be present throughout the course.

*Note: The use of GFP from a recombinant source (E. coli) is also being
used
 as starting material due to its popularity within the scientific
community.

For further details you can reach us,

by E-mail at:  meton at rci.rutgers.edu
by phone at:  (732) 932-9071 extension 225
by FAX at:  (732) 932-3633

for a brochure and further information please visit the GFP purification
short course official Web site at:
http://www.rci.rutgers.edu/~meton/protein.html

Notice: We will be discounting the registration fee by 20% for anyone
registering via the WEB!






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