Domain of GFP responsible for fluorescence

Wolfgang Schechinger Wolfgang.Schechinger at med.uni-tuebingen.de
Sat Jun 3 10:57:30 EST 2000


All you may delete, is some 5 aminino acids at the c terminus. the 
fluorochrome ist formed from three aa around position 65 and hosted 
in a hydrophobical cage formed from the rest of the protein. maybe 
you are able to get hold of one of these nice pictures showing GFP's 
barrel structure, it is almost self explanatory.

What's the reason you want to delete these 55 AAs'?

Wolfgang

> Does anybody Know what region of the smGFP is responsible for the
> fluorescence? More specifically, would an N-terminal 55 amino acid
> deletion still fluoresce?  Any help would be appreciated.
> 
> 
> Jared LeMaster
> Plant Biotech Center
> The Ohio State University
> 
> 
> 
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Dr. Wolfgang Schechinger, Dept. of Pathobiochemistry
University of Tuebingen, Germany
email: wolfgang.schechinger at med.uni-tuebingen.de 
wwWait: http://www.medizin.uni-tuebingen.de/~wgschech/start.htm
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