New Chomogenic / fluorogenic Glucuronide Substrate

Damian Bond damian.bond at virgin.net
Sun Jun 18 05:36:29 EST 2000


We have a novel chromogenic Glucuronide substrate. It performs in the same
way as X-Glucuronide, but can be made to either a) fluoresce or b) give an
intense black colouration that binds tightly to the bacterial colony.  I am
trying to find new applications for it.

We are already incorporating it into microbiological media for E.coli, such
as TBGA (LAB M)
or TBX (Oxoid), but its real strength appers to be in a media with a mix of
New-Glucuronide and X-Gal, giving a E. coli / Coliform media (black
coliforms and blue E. coli - if I can remember my enzyme pathways correctly
at this time of night).

I am trying to find other uses for the substrate. I hear claims that
Glucuronide substrates are used in other fields, such as GUS reporter genes
in Plant but I cannot get any confirmation on this. Basically, if I am able
to increase the use, the volumes increase and the synthesis costs decrease,
allowing the substrate to be made available at a sensible price.

Does anyone know of other applications or have an interest in collaborating
to explore uses of this substrate ?

--
Damian Bond
Managing Director
IDG (UK) Ltd

e-mail dbond at idgplc.com

--
Damian Bond
Tel 01706 872 775
Mobile 07798 600 833
e-mail damian.bond at virgin.net



--
Damian Bond
Tel 01706 872 775
Mobile 07798 600 833
e-mail damian.bond at virgin.net







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