DsRed expression in E. coli

Isabelle Sagot isabelle.sagot at urz.uni-heidelberg.de
Mon Jun 19 09:54:50 EST 2000


Hi,

Does anybody have been successfull in overexpressing DsRed in E. coli ?
If yes, what were the expression conditions ? (how many time induction,
temperature of induction, E coli strain....).
I have also problems for cloning the DsRed coding sequence (from the
clontech plasmid pDsRed-N1).
After cloning in my vector, the restriction map is OK but there is no
fluorescence (Microscope or spectrofluorimeter checking).
Any help is welcome.

Thank you very much

Isabelle

=====================================
Dr. Isabelle Sagot
Biochemie-Zentrum Heidelberg
AG Hurt, 4. OG
Im Neuenheimer Feld 328
D-69120 Heidelberg
Tel: +49-(0)6221-54-4176
Fax: +49-(0)6221-54-4369
Email: hn7 at ix.urz.uni-heidelberg.de
WWW:www.uni-heidelberg.de/zentral/bzh
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