nonfluorescent GFP fusion

Andreas Elsing elsing at
Tue Jun 27 08:01:01 EST 2000

arndt_holger.brachat at wrote:

> Dear GFP fans,
> has anyone ever heard of a protein/peptide that when fused to GFP  PREVENTS  GFP
> fluorescence?



to my knowledge it happens quite frequently that GFP looses fluorescence when fused
to a protein.
To do so its just "try and error".
What I know for sure is that GFP fluorescence can remarkably decrease after fusion
(happend to me more than once)

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