nonfluorescent GFP fusion

Andreas Elsing elsing at lmb.uni-muenchen.de
Tue Jun 27 08:01:01 EST 2000


arndt_holger.brachat at pharma.Novartis.com wrote:

> Dear GFP fans,
>
> has anyone ever heard of a protein/peptide that when fused to GFP  PREVENTS  GFP
> fluorescence?

<schnipp>

Hi,

to my knowledge it happens quite frequently that GFP looses fluorescence when fused
to a protein.
To do so its just "try and error".
What I know for sure is that GFP fluorescence can remarkably decrease after fusion
(happend to me more than once)

Best
A.Elsing
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