stable transfection

Soenke Behrends behrends at plexus.uke.uni-hamburg.de
Wed Mar 8 15:42:14 EST 2000


Dear netters,

we would like to stably transfect PC12 cells with 
an EGFP fusion protein using the Clontech pEGFP
vector and Geneticin as selection antibiotic. 
Transient transfections work nicely and 
we get nice green fluorescence. 

Is it true that it is better to linearize the vector for
stable transfection protocols and if so where should
we cut the vector? 

We would be very happy about a protocol that
has worked well in a similar setting. 

Thanks for your time and help
Soenke 




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