cotransfect or bicistronic?

David N. Levy levy at removeforspam.uab.edu
Fri Mar 10 17:01:58 EST 2000


In article <38c958cb.6706726 at news.uni-hamburg.de>
behrends at plexus.uke.uni-hamburg.de (Soenke Behrends) writes:

> 
> Dear netters,
> 
> Is it fair to say that when you transfect pEGFP with green
> fluorescence only and another vector with the same features
> with your gene of interest that the fluorescence leads you
> with some likelihood to the cells expressing you gene of
> interest (e.g. for electrophysiology)?
> 
> i have heard (but do not have good scientific reference)
> for the notion that when you transiently transfect cells 
> (say with 10% of cells being transfected) you mostly 
> get the same 10% of cells cotransfected with the another
> similar vector. 
> 

Yes, this is true.  I have cotransfected EGFP and EYFP vectors, and by
far most cells which express one color also express the other.

David N. Levy, Ph.D.
University of Alabama at Birmingham
Birmingham, AL 25294




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