Stabilising GFP in mouse embryos

[Loughna, Siobhan]

I need help!  I am analysing GFP which is cytoplasmically located in E9.5
mouse embryos.  When I fix the embryos (4% PFA with or without 0.1%
glutaraldehyde, for various times) and analyse from 1-3 days later the images
are blurred and the GFP is diffuse - if I analyse on day of isolation, the
images are great.  Can anyone help me on what I can do to stabilise the GFP?

Thanks in advance.


Siobhan Loughna
Cardiology Labs
5323 Harry Hines Blvd., NB11.200
University of Texas and Southwestern Medical Center at Dallas
Dallas, Texas 75235-8573, USA.

Tel# 214 648 1419
Fax # 214 648 1433
e-mail  loughna at ryburn.swmed.edu
---