Extraction of GFP protein

Chris LaRosa clarosa at biocomp.unl.edu
Tue Mar 14 14:36:56 EST 2000


We simply lyse the cells with 1 % SDS, as the protein will fluoresce in SDS..
Simple.

Sebastien Paris wrote:

> Hi GFPers,
>
> Does anybody know how to extract GFP proteins from a cell culture or tissue
> in order to measure the fluorescence with a fluorimeter ?





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