GFP as nucleo-cytoplasmic transport marker

[Felix Bestvater]

we've tagged our gene of interest with two different GFPs at both 
sequence termini simultaneously in order to exclude nuclear import 
caused by passive diffusion. the complete protein should now be about 
84 kDa and is still accumulated inside the nucleus 24h after 
transfection. according to the literature proteins with more than 68 
kDa should not be able to get into the nucleus by passive diffusion 
at all. 

is it thinkable that the GFPs are faided in through the 
nuclear pore consecutively like pearls of a pearl necklet? does 
anyone have experience with it?

thank you for any help

Felix


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Felix Bestvater
Deutsches Krebsforschungszentrum
Abt. Biomedizinische Strukturforschung (A0600)
Im Neuenheimer Feld 280

D-69120 Heidelberg

TEL:   (+49) 06221 - 42 34 32 (33)
Fax:   (+49) 06221 - 42 34 59
Email: F.Bestvater at dkfz-heidelberg.de
WWW:   http://www.dkfz-heidelberg.de/a0600/
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