Can EGFP appear yellow ?

Dr Robert Gay at
Wed Mar 22 00:42:15 EST 2000

Hi GFPers

I've noticed that when I have CHO cells highly expressing EGFP - that is
greater than 10^3 on the FACS - they appear yellow when viewed on the
fluorescent microscope.

Has anyone else come across this phenomenon ?  Does anyone have an
explanation ?



Dr Robert Gay

Post Doctoral Research Fellow
CRC for Biopharmaceutical Research
Department of Biotechnology
University of New South Wales
Sydney, NSW 2052, Australia

Tel : +61(0)2-9385-1892
Fax : +61(0)2-9313-6710


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