Soluble Fluorescent Proteins via Sf cells

James E Pease owls at owls.screaming.net
Thu Mar 30 14:15:31 EST 2000


Dear Newsgrop,

Has anyone out there succesfully expressed a small cytokine or other soluble
protein (8-10 kDa) to which GFP has been fused at the C-terminus?  I intend
to use Sf21 cells and utilise the native cytokine signal peptide to get the
protein into the supernatant.

Many thanks,

James






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