Enzyme kinetics using GFPs

JJ urbane45 at netscape.net
Sat May 6 14:52:27 EST 2000


Hello,

    I would like to determine the kinetics of an enzyme in living cells
    using mutant GFPs as the reporter. I would like to monitor the catalytic
    activity of the enzyme. I am very much interested in being able to
    extract quantitative data about the enzymes activity.

    Does anyone know of any research along these lines using GFPs?

    Any help would be most helpful.

    Thank you,

    JJ
urbane45 at netscape.net

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