GFP tagged on both sides

John Doe John at doe.nowhere
Thu Nov 9 09:38:14 EST 2000


Hi,

In <Pine.A41.4.21.0010051321080.89938-100000 at homer06.u.washington.edu> 
at 5 Oct 2000 21:25:33 +0100, 'Mike' Michael J. Moser wrote:
>Andrej
>
>There is a paper from Roger Tsiens lab where they fused two
>spectral variants of GFP to calmodulin.  The resulting molecule was able
>to serve as a measure of intracellular Ca2+ by FRET.  See:
>
>Nature 1997 Aug 28;388(6645):882-7 Related Articles, Books, LinkOut 
>
>Fluorescent indicators for Ca2+ based on green fluorescent proteins and
>calmodulin.
>
>Miyawaki A, Llopis J, Heim R, McCaffery JM, Adams JA, Ikura M, Tsien RY
>
>Happy GFPing,
>
>Mike Moser, Ph.D.
>Department of Pathology, University of Washington                  
>Health Sciences Building, 1959 N.E. Pacific, Room K-065
>Box 357705, Seattle, WA  98195-7705
>Tel: 206.543.6585
>FAX: 206.543.3967
>moser at u.washington.edu
>http://faculty.washington.edu/moser
>
>On Thu, 5 Oct 2000, Andrej.Michalik wrote:
>
>> Dear netters,
>> I wonder whether anyone ever produced GFP (or EGFP) fused to a protein at
>> each end - "internal GFP", and whether it stayed fluorescent? Is it
>> trial and error or should I better not try it?
>> Thanx
>> Andrej
>>  
>> 
>> 
>> ------------------------------------------------------------------------------
>>         A               andrej michalik              
>>        A A              department of biochemistry 
>>       A   A             university of antwerp (uia)
>>      A     A            universiteitsplein 1
>>     AAAAAAAAAAAAA       B-2610 antwerpen  
>>    A         AAA        belgium
>>   A           A         tel: +32-(0)3-8202642    
>>                         amichal at uia.ac.be
>> ------------------------------------------------------------------------------
>>      
>>      
>>    
>>   
>>  
>> 
>> 
>> 
>
>
>
>---


----
John Doe






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