Fixation and storage for FACS

Marcus Watkins mwatkins at cyllene.uwa.edu.au
Tue Jan 16 19:59:08 EST 2001


Hi


I am hoping to fix EGFP expressing cell culture lines for FACS analysis
and store them until I can analyse them. By the looking through the
archive it looks like paraformaldehyde fixation is the best, but how
long can I store cells in this solution?

Basically I'd like to know what is the best fixation technique and how
long I can store cells with the minimum loss of fluorescence.  

Thanks

Marcus
-- 
"If at first you don't succeed, try, try, again. Then quit. There's no
use in being a damn fool about it." --W.C. Fields

Marcus Watkins
Postgraduate Student
Centre For Applied Cancer Studies/Lions Cancer Institute
University of Western Australia


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