FLAG TAG DETECTION

Kathryn Sunn k.sunn at garvan.org.au
Thu Mar 29 22:35:57 EST 2001


Dear All,

I was wondering if anyone knew if 1 FLAG tag epitope was enough to be
detected by indirect immunofluorescence in a transfected cell line?? My
expression construct only has 1 tag and I am having difficulty detecting
the epitope, however my constructs with 3 or more tags seem fine.


Thanks in advance


kate

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Kathryn Sunn  BSc (Hons)
Bone and Mineral Research Program
Garvan Institute of Medical Research
St Vincent's Hospital                          Tel:   (02) 9295 8260
Sydney NSW 2010                                Fax:   (02) 9295 8241
Australia                          email: k.sunn at garvan.org.au
___________________________________________________________________



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