FLAG TAG DETECTION

Cornelius Krasel krasel at wpxx02.toxi.uni-wuerzburg.de
Fri Mar 30 14:32:36 EST 2001


Kathryn Sunn <k.sunn at garvan.org.au> wrote:
> I was wondering if anyone knew if 1 FLAG tag epitope was enough to be
> detected by indirect immunofluorescence in a transfected cell line??

I have been successful in detecting single flag-tagged beta2-adrenergic
receptors with Sigma's M2 antibody and a secondary alexa-labeled anti-mouse
antibody from Molecular Probes. Expression level of the receptors is around
1 pmol/mg membrane protein. Similar observations have been reported by a
number of receptor people.

--Cornelius.

-- 
/* Cornelius Krasel, U Wuerzburg, Dept. of Pharmacology, Versbacher Str. 9 */
/* D-97078 Wuerzburg, Germany   email: krasel at wpxx02.toxi.uni-wuerzburg.de */
/* "Science is the game we play with God to find out what His rules are."  */




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