Hoechst 33342

Ulrich Huth ulrich.huth at pharmazie.uni-freiburg.de
Mon Apr 22 01:46:36 EST 2002


Dear all,

I want to buy Hoechst 33342 to stain my living COS-7 cells. (DAPI staining
does not work).

Is there anybody having experience with this dye? Molecular Probes does not
provide a

protocol for nuclei staining. Which concentrations do I use? What is the
incubation time?

Any problems or tricks with this fluorescent marker? Do you think the
Hoechst Dye is

a good marker?



Thank you in advance,



Ulrich Huth



Ulrich.huth at pharmazie.uni-freiburg.de

=====================================

Ulrich Huth
Albert-Ludwigs-Universitaet
Pharmazeutische Technologie
Stefan-Meier-Str.19
79104 Freiburg
Germany
Tel: 0761-203 4899
Fax: 0761-203 6326





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