Questions ?

DAVIDS, L, LESTER, MR lester at uctgsh1.uct.ac.za
Tue Apr 23 10:03:25 EST 2002


Hi All,

I am doing flow cytometry on a Becktin-Dickinson FACStar and am 
looking at a GFP-fusion protein located in mitochondria. I extract 
the mt by standard protocol but then do not get very good 
fluorescence on the FACS machine. Does anyone have any suggestions ? 
Also, what is the PMT values likely to be in FL-1 (FITC channel) to 
view the GFP-fusion protein ? I am using HepG2 cells and generally 
transfect 1x60mm plate of cells.

Looking forward to a reply.
********************************************
Lester M. Davids,M.Sc(UCT)
Lennox Eales Porphyria Laboratories
MRC/UCT Liver Research Centre
K-FLoor, Old Groote Schuur Hospital
UCT Medical School
Observatory, 7925
South Africa
Tel. 27-21-406 6519
Fax. 27-21-447 9670
:-) "F>R>O>G!" :-)
********************************************

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