[Fluorescent-proteins] pHygEGFP or pNeoEGFP

Albert Jordan albert.jordan at crg.es
Mon Apr 10 09:50:19 EST 2006


Hi,
does anybody has a good or bad experience with any of these plasmids 
expressing fusion proteins between EGFP and Neo or Hyg resistance. 
Are both markers well expressed, in what cell lines, any toxicity... 
I'm interested in using them in Jurkat cells after cloning the fusion 
protein into a retroviral vector, under the control of the retroviral 
promoter. Thanks in advance,
Albert



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