From biochem2 from gmx.de  Wed Mar  7 09:48:23 2007
From: biochem2 from gmx.de (biochem2@gmx.de)
Date: Wed Mar  7 10:01:09 2007
Subject: [Fluorescent-proteins] stability of luciferase reagents?
Message-ID: <1173278903.252218.214590@64g2000cwx.googlegroups.com>


Hi there,
I am a PhD student in Dortmund/Germany and would like to set up a
luciferase assay- does anyone have experience how to solve the
reagents (luciferase, D-luciferin sodium salt), how stable they are in
solution and if you can freeze stock solutions? And how stable the
bioluminescent signal is?
Thanks a lot in advance!

Uyen

From piercarlo.montecucchi from tin.it  Thu Mar  8 04:48:39 2007
From: piercarlo.montecucchi from tin.it (piercarlo.montecucchi)
Date: Thu Mar  8 09:40:42 2007
Subject: [Fluorescent-proteins] Re: stability of luciferase reagents?
References: <1173278903.252218.214590@64g2000cwx.googlegroups.com>
Message-ID: <45efd91a$0$4790$4fafbaef@reader4.news.tin.it>

You could read the experimental procedures in one of my old papers : "
Stimolazione della formazione di AMP ciclico nel cervello di ratto "in
vitro" da parte della nicergolina" - Il Farmaco 31, 10-17 (1976)

Regards
PCM