[Fluorescent-proteins] Green Fluorescent Proteins

[Mahima Laroyia]

HI , 

I was reviewing the site and got to know some interesting facts about green fluorescent proteins but need to explore more. Can one answer my following questions ? 

1.  In what all case using green fluorescent protein to study gene expression fails ?

2. What are the developments that are still in progress to make GFP or its derivative better but are not published yet ? 

Waiting for your replies 

MAHIMA