HELP - Ethidium Bromide contamination

T Partridge mb2tp at silver.shef.ac.uk
Mon Dec 5 12:07:12 EST 1994


Maniatis doesn't really have any information on how to decontaminate 
people - only benches - which involves KMnO4, 2.5N NaOH and 2.5N HCl.  
Given the dilution of the Ethidium bromide (assuming a 10mg/ml solution, 
1 ml in 1 l is 10 ug/ml) I think that washing with copious water is about 
the best possible precution.  Effects will probably take years to become 
apparent, and I have no idea how carcinogenic EtBr is compared too other 
lab chemicals.   A supervisor of mine once injected some into his finger 
while trying to unload a CsCl gradient, and we just washed that under 
cold water to get it to bleed as much as possible.   

Toby

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