CA repeat and linkage?
roger at kean.ucs.mun.ca
Mon Oct 17 07:01:21 EST 1994
In article <yd128-1510941419210001 at mclab.bio.albany.edu>, yd128 at albnyvms.bitnet (Dr. YL Du) writes:
> We are sequencing a piece of mouse genome DNA and find some CA repeats,
> one is 24 bp (ie. 12 CA repeat), and another is 30 bp. Can we base on
> these two CA repeats to locate the position of this piece of genome to a
> specific chromosome? Welcome any suggestion.
Since there are thousands of copies of CA repeat sequence scattered
throughout the genome, you should instead determine the sequence of DNA
flanking the repeat element. Check the DNA databases to make sure it is
unique (single-copy) sequence.
Then use that sequence to design PCR primers. These can be used to
screen chromosome-specific DNA libraries.
Alternatively, the sequence information could be used to design
a probe for Fluorescent In Situ Hybridization, which will show you the
chromosomal position of your clone.
Roger C. Green, Faculty of Medicine Phone: (709)737-6884
Memorial University , St. John's, Newfoundland FAX : (709)737-7010
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