LOH analysis from paraffin block DNA

rifat_h rifat_h at icr.ac.uk
Tue Nov 21 14:13:38 EST 1995


Hi,

	Does anyone have any good PCR cycling methodology to amplify DNA obtained
from paraffin blocks or from tissue slides.

I am trying to do a loss of heterozygosity study on DNA obtained from slides using
ABI GeneScan software and am having troubles in getting all the flourescent markers
to work. If anyone knows of any references or can advice on this matter then I
would be very grateful.

Thanks.


rifat_h at icr.ac.uk




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