DNA ligation help

chunlin xin xcl at med.unc.edu
Sat Jun 1 10:27:52 EST 1996


Hi, there, I have trouble in DNA ligation, the case below:
          Insert Fragment: 16.3kb SalI Frag.
                   Vector: Bscrip-KS(-) SalI cut plasmid or other 
                           salI cut expression vector
          It looks like no ligation problem, but in fact, I cannot 
success to ligate them and transfer them into DH5a no matter I use 
chemical or electroparation method, Have anybody  ever meet the same problem?
How can I solve them? Any suggestion is appreciated! Thanks for you attention!



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