isozyme analysis using agarose gels

David L. Robinson dlrobi02 at HOMER.LOUISVILLE.EDU
Wed Nov 6 21:20:24 EST 1996


Does anyone have any experience doing native-protein electrophoresis and 
enzyme activity staining using horizontal agarose gels (instead of 
vertical PAGE)?

I feel like this would be a easier, cheaper, and safer technique for 
undergraduates to do isozyme analysis but am not sure of the 
feasibility. Even a reference on this procedure would be great.

Thanks,

Dave Robinson
Biology Dept.
Bellarmine College
Louisville, KY



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