D5S392

Robyn Wallace rwallace at mad.adelaide.edu.au
Wed Nov 27 20:27:07 EST 1996


I have used D5S392 in the past with no problems (using the same primers as
Fleming described).  I am however using 32P to detect the product.

My PCR conditions are

94C 60 sec
60  90      } 10 cycles
72  90

94C 60 sec
55  90      } 25 cycles
72  90

Mg = 1.5mM

I know the length of the cycles is a bit of an overkill but I use these
conditions to amplify all my AC repeats.  I'm not sure if these conditions
will be suitable for fluorescent detection though.

Robyn




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Robyn Wallace
Department of Cytogenetics and Molecular Genetics
Womens and Childrens Hospital
North Adelaide, South Australia  5006
Ph  61 08 204 6442
Fax 61 08 204 7342
Email  rwallace at mad.adelaide.edu.au

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