Microsatellite stutter bands

John Ladasky ladasky at leland.Stanford.EDU
Wed May 21 03:20:51 EST 1997


Greetings,

	I'm doing a little microsatellite typing on the side of my regular
project.  I'm dismayed to see several prominent secondary products.  My
understanding is that these secondary peaks, called "stutter bands," arise
from slipped-strand mispairing during PCR.  A colleague said that using *two*
temperature thermal cycling (anneal and extend at the same temperature) is
supposed to help reduce the stutter bands, but he didn't remember the refer-
ence.  Can anyone vouch for this and/or remember the reference?  Do you have
any other hot tips for cleaning up microsatellite PCR?  Thanks!

-- 
Unique ID : Ladasky, John Joseph Jr.
Title     : BA Biochemistry, U.C. Berkeley, 1989  (Ph.D. perhaps 1998???)
Location  : Stanford University, Dept. of Structural Biology
Keywords  : immunology, music, running, Green



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